1. The gene for a eukaryotic polypeptide hormone was isolated, cloned, sequenced
ID: 55391 • Letter: 1
Question
1. The gene for a eukaryotic polypeptide hormone was isolated, cloned, sequenced, and overexpressed in a bacterium. After the poly peptide was purified from the bacterium, it failed to function when it was subjected to a bioassay in the organism from which the gene was isolated. Speculate why the recombinant DNA product was inactive.
2. You wish to clone a yeast gene in Lambda Phage. A) Briefly explain how this would be accomplished. B) Why is it desirable to cleave both the yeast DNA and the Lambda Phage DNA with the same yeast DNA and theLambda Phage DNA with the same restriction enzyme?
Explanation / Answer
lambda phage are vectors that infect bacteria. They have high transformation effficiency.They complete the life cycle even if the foreign DNA is inserted in its genome.
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