ill Verizon LTE 11:52 AM * 96%. Quiz 4: Attempt 1 Rhodamine G is a popular fluor

Question

ill Verizon LTE 11:52 AM * 96%. Quiz 4: Attempt 1 Rhodamine G is a popular fluorescent tag and it absorbs light at 475 nm. A series of fluorescence emission experiments were performed to examine the emission characteristics of rhodamine and one data point is given below. What is the most likely reason the emission intensity zero? Experiment excitation (nm) emission (nm) [Rhodamine]/ mg/mL Emission Intensity (Unitless) 1475 425 0.01 0.0 The intensity is being measured at a shorter 1)wavelength than excitation so no emission would be seen The sample concentration must be above the self quenching concentration of Rhodamine 2) Rhodamine releases all of the absorbed energy through non-radiative processes. Rhodamine is not a suitable molecule for a fluorescence experiment 0 of 5 completed Save for Later Submit

Explanation / Answer

In emissive molecules, the emission wavelength is always greater than absorption wavelength because some of the energies of the exciting photon are dissipiated as heat during non-radiative transitions. Hence emission should be recorded at longer wavelengths than excitation. So the most likely reason for zero emission intensity is option (1).

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