Lab URL: http://www.mhhe.com/biosci/genbio/tlw3/virtual_labs/lab11/labs/lab11/ex

Question

Lab URL: http://www.mhhe.com/biosci/genbio/tlw3/virtual_labs/lab11/labs/lab11/exp3/index.html

Lab Overview: http://www.mhhe.com/biosci/genbio/tlw3/virtual_labs/lab11/labs/lab11/resources/overview.pdf

1.) In a continuous culture, where CV103, CV101, and CV116 are all cultured together, CV`03 out competed CV116 even though there was glycerol present in that culture media (see observation #5). Why was CV116 able to out compete CV103 in this experiment when 1mM of glycerol was added to the glucose-limited medium?

2.) Might the results be different if the supple of glucose was not limited?

3.) Do your results support or reject your hypothesis?

Explanation / Answer

This experiment deals with the fact that de novo mutations can give rise to bacteria having a novel genetic makeup/polymorphism.

1) The experiments suggest that CV116 utilizes glycerol as an energy source and thus out competed CV103 when 1mM glycerol was added in a glucose-limited medium. CV103 was glucose dependent and hence on supplementing glycerol CV116 out grew CV103.

2) If supply of glucose was not limited than CV103 would continue to out grow CV116 as glycerol would be limiting.

3) These results support the hypothesis that new mutations in bateria lead to polymorphisms that help them carve a different nieche for themselves which help in their survival.

"A malaria biologist"

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