because all DNA polymerase a synthesize DNA in the 5\' to 3\' direction, and the

Question

because all DNA polymerase a synthesize DNA in the 5' to 3' direction, and the parental strands are antiparallel, DNA replication is accomplished with the use of 2 mechanisms: continuous and discontinuous replication. Because all DNA polymerases synthesize DNA in the 5'-to-3' direction, and the parental strands are antiparallel, DNA replication is accomplished with the use of two mechanisms: continuous and discontinuous replication. Indicate whether the following items relate to (1) continuous replication, (2) discontinuous replication, or (3) both modes of replication. primase single-strand binding protein sliding clamp RNA primers _ -leading strand lagging strand Okazaki fragments DNA helicase DNA ligase

Explanation / Answer

Actually DNA replication is sometimes labeled as semi-discontinuous; where the leading strand is repliacted in a continuous process & the lagging strand is replicated in a dis-continuouus process.

Primase- both ( both the replication of leading & lagging strands need a RNA primer for initiation (lagging strands need multiple primers for replication) ; RNA primers are synthesized by Primase)

Single stranded binding proteins - both ( SSBP stabilize the ssDNA during replication)

Sliding clamp- both (it allows DNA polymerase to bind tightly to the template DNA strands & prevent dissociation of these enzymes)

RNA primers - both [both the replication of leading & lagging strands need a RNA primer for initiation (lagging strands need multiple primers for replication)]

Leading strand - continuous replication

Lagging strand- discontinuous replication

Okazaki fragments - discontinuous replication ( The lagging strand is synthesized by the joining of ~200 to 1000 nucleotide long fragments called Okazaki fragments)

DNA helicase- both (it is required for the unwinding of the parental ds DNA to ssDNA which will act as template for both the lagging & leading strand replication)

DNA ligase- discontinuous replication ( needed for the joining of Okazaki fragments)

( although after DNA replication is done, the RNA primers are removed by Nick translation; then the gaps are sealed by DNA ligase- going with this mindset, it is required for both)

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