g Ten reaction mixtures each containing the same concentration or enzyme were ma

Question

g Ten reaction mixtures each containing the same concentration or enzyme were made up to various substrate concentrations and the initial velocities were determined as shown in Table 7-3. Using the Lineweaver-Burk equation, graphically determine. K and Vmax Note that one of the critical factors in the accuracy of this determi- nation is the scales selected for the ordinate and the abscissa. What range of substrate concentrations are most useful for these deter- minations? Table 7-3. Initial rates at yarious substrate concentrations for a hypothetical enzyme-catalyzed reaction (Problem 7-9) [S] (moles/iter) o (umoles/min) 1.0 X 10-3 6.0 X 10-i 1.0 X 104 6.0 x10-5 3.0 X10-s 2.0 X 10S 1.0 X 10S 6.0 X 106 1.0 X10-6 5.0 X 10-7 65 63 61 42 27 17 9.5 2.2 1.1

Explanation / Answer

Here, we have [S] values. We convert them to 1/[S] and v values to 1/v values.

Plotting 1/[S] vs 1/v,

we get, vmax= 1/0.015 = 67 umol/min

km= 3*10-5 m

1/v 1/[S] 0.0154 1000 0.0159 2000 0.0196 10000 0.0238 20000 0.0303 33333 0.037 50000 0.0588 100000 0.1053 200000 0.4545 1000000 0.9091 2000000

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