in human blood? A sample has an absorbance of 0.15 at 675, what is its concentra
ID: 546511 • Letter: I
Question
in human blood? A sample has an absorbance of 0.15 at 675, what is its concentra tion (mM) based solely on the calibration graph in Figure below? 2. 1.2 Concentration (mM) Figure Calibration Curve at 675 nm Questions 3 and 4 refer to the absorption spectrum for a 0.40 M solu- tion of metal ion M* and a calibration curve for the metal ion M. Absorption Spectrum of 0.40 M M+ Calibration Curve for M* 0.8 0.7 0.6 1.2 0.8 0.6- 0.4+-r-T- 0.3 0.2 400 450 500 550 600 650 700 (A) nm 0 0.1 0.2 0.3 0.4 0.5 M'] (Molar) 3. At what wavelength was the calibration curve obtained? 4. A sample of this solution of unknown concentration of M has an absorbance reading that is "off scale" when read at the wavelength that was used to construct the above calibration curve. You dilute the sample by adding 5.0 mL of water to 1.0 mL of the solution of M. The absorbance of the diluted sample when read at the wavelength of the calibration graph = 0.35, what is the concentration (M) of the original undiluted sample? Experiment 3-3 What Factors Affect Color IntenExplanation / Answer
Q2.
A = e*l*C
A = absorbance of sample
e is the molar absorptivity , the typical units are 1/M-cm
l size of cuvette, typically reported in cm
c is the molar concentration, in mol per ltier or M
then...
A = el*C
at A = 0.15
concentration is 0.30 mM approx
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