The number of cells in a culture is directly proportional to the absorbance. Com

Question

The number of cells in a culture is directly proportional to the absorbance. Complete the following statements with "equal to", "greater than", or "less than": Upon starting you put Cultures A and B in the incubator for 15 minutes before putting Cultures C and D into the incubator and starting your timer. After the first 20 minutes culture A and B densities would be Culture C and D densities. If you pipet inaccurately so that 0.2 ml more cells were added to Culture B than Culture A at time zero, the absorbance reading of Culture A would be Culture B at early time points. If each culture starts with the same cell number, the absorbance readings should be Culture A at time 0. If you forget to add the antibiotic in Culture C, the absorbance readings at 20 minutes should be the absorbance readings at 120 minutes.

Explanation / Answer

a. Greater than

b. less than

c. equal to each other

d. Different conditions applied here. First, Antibiotic is not growth factor, so if you add or may not add the absorbance with respect to cell density will increase with an increase in time point and cell proliferation.

Therefore, at 20 min the absorbance is lesser than 120 min.

Lack of antibiotic may lead to contamination, so we need to cross check this event before going to absorbance checking.

Related Questions

Navigate

• Browse (All)
• Browse T
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.