ng Learning An oligomeric protein has a molecular weight of 232000. A 830 mg sam

Question

ng Learning An oligomeric protein has a molecular weight of 232000. A 830 mg sample was treated with an excess of were h 1-fluoro-2,4-dinitrobenzene (Sanger's reagent) until the reaction was complete. Next the peptide bonds yzed by heating with concentrated HCI. The product of the complete hydrolysis contained 3.2 of the compound shown below, and no other 2,4-dinitrophenyl derivatives of the a-amino groups of other amino acids could be found. NO2 How many polypeptide chains are in this protein? Enter your answer as a whole number). NH-C-COOH HC -CHa Number CH3

Explanation / Answer

mmoles of 2,4-dinitrophenyl derivative = mass / molar mass

                                                          = 3.2 / 256

                                                           = 0.0125 mmol

mmoles of protein = 830 / 232000 = 3.58 x 10^-3

number of poly peptides bonds = 0.0125 / 3.58 x 10^-3

                                                  = 3.49

number of poly peptides bonds = 3.0

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