Question 13 of 17 Mapoob Sapling Learning The table below shows the data for pur

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Question 13 of 17 Mapoob Sapling Learning The table below shows the data for purification of a hypothetical protein. Complete the table by ng in the missing values for the specific activity, yield, and purification level Step Total Protein Total Activity Specific Activity Yield Purification (mg) 14900 4480 (units) 155000 139000 (units mg-1) 10.4 31.0 level (%) 100 89.5 Number Homogenization Salt fractionation 2.98 Number Number lon-exchange chromatography 1240 118000 Number Number Number Gel-filtration chromatography 69.3 77300 Number Number Number Affinity chromatography 4.16 58100 Note: A red X willappear in the upper left corner of any box that has an incorrect answer If a box does not have a red X at the upper left, that particular answer is correct.

Explanation / Answer

Specific Activity (mg-1)

118000/1240= 95.16

Yield (%)

(118000/139000)*100 = 84.9

Purification Level

95.16/31 = 3.06

Ion -Exchange Chromatography

Specific Activity (mg-1)

118000/1240= 95.16

Yield (%)

(118000/139000)*100 = 84.9

Purification Level

95.16/31 = 3.06

Gel-Filtration Chromatography 77300/69.3 = 1115.44 (77300/118000)*100 = 65.50 1115.44/95.16 = 11.72 Affinity Chromatography 58100/4.16 = 13966.34 (58100/77300)*100 = 75.16 13966.34/1115.44 =12.52

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