Question
HW 4. Sdent C needs to separate two compous fractions, and he shows you the TLC of the fractions from the coloue Consider t m one unother and runs a coiomn tafter runsing his column, he collected 5 c ten calls you o.er indesplans Ebn A Student C loaded his sumple oate the column diluted witls too mach solvenst Would this scenario lead to the above TI.C result? Yes or No. Why? B. Student C used too nonpolar an eluent s stem w hen running the column. Would this scenario lead to the above TLC result? Yes or No. Why? C. Student Cused too polar an eluent system when running the column. Would this scenario lead to the above TLC result? Yes or No. Why? Student C used too little adsorbent when running the eolumn. Would this scenario lead to the above TI.C resulh? Yes or No, Why? D. E. Student C added eluent prior to material adsorbed into silica when running the column Would this scenario lead to the above TLC result? Yes or No. Why? F. Student C used a column that had too large a diameter. Would this scenario lead to the above TLC result? Yes or No. Why? 74
Explanation / Answer
Answer A) Yes as the solvent is more therefore voltalization takes place
Answer B) Yes this wil effect the result as it contain non-polar eluent system and it will also effect the retention factor
Answer C ) No this wil not effect the result as it contain polar eluent system
Answer D) NoToo little adsorbent does not affect the TLC result or retention factor
Answer E) Yes this will effect the retention factor as well the result of TLC
Answer F) No this will not effect the Result
