The chemical intermediates A, B, C, D & E all occur in the same biochemical path
ID: 54278 • Letter: T
Question
The chemical intermediates A, B, C, D & E all occur in the same biochemical pathway. Chemical F is the product of the pathway. Mutants 1 through 5 are all F- (i.e. these auxotrophic mutants CANNOT produce chemical F). The following table denotes which intermediates will promote growth in each of the mutants; ARRANGE the intermediates in order of their occurrence in the pathway. A plus (+) indicates growth and 0 indicates lack of growth.
Mutant
A
B
C
D
E
F
Wild-type
+
+
+
+
+
+
1
+
+
+
0
+
+
2
+
+
+
0
0
+
3
+
0
+
0
0
+
4
0
0
+
0
0
+
5
0
0
0
0
0
+
Mutant
A
B
C
D
E
F
Wild-type
+
+
+
+
+
+
1
+
+
+
0
+
+
2
+
+
+
0
0
+
3
+
0
+
0
0
+
4
0
0
+
0
0
+
5
0
0
0
0
0
+
Explanation / Answer
Based on the given data,
None of the mutants can grow on minimal media and the mutant, which use less number of intermediates for survival is must be placed last in the pathway. The mutant, which use more number of intermediates for survival is must be placed first in the pathway.
Thus, the biochemical pathway is as follows: D-->E-->B-->A-->C-->F
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