Need Help with question one, three and four please! (Please provide photos of di
ID: 54215 • Letter: N
Question
Need Help with question one, three and four please! (Please provide photos of diagrams/maps!)
QUESTION 1
1. Consider temperate phage ?? (a.k.a. “Fifi”). See the blue map of ?? DNA as isolated from the phage capsid below. Like phage ?, ?? integrates via its attP into a unique attB in the E. coli chromosome, located between genes required to synthesize adenine and genes required to synthesize leucine (red map is region of host chromo where ?? inserts). Note that attB is located much closer to leu than to ade. ?? possesses a cluster of genes essential for phage production (= A B C) and a cluster of non-essential genes (= 1 2 3).
Assuming that the attachment sites pair in the orientation shown in the above figures, draw the prophage map for ??) . Your map must include ALL sites annotated in the above figures.
QUESTION 3
Assume that you have at your disposal various deletion mutants of the phage ?? and host genomes. Chose from the deletion list below those that can aid the recovery of ?? ade+ transducing phage. Explain your choice(s).
?? deletions: E. coli host deletions:
attP ade
A B C attB
1 2 3 leu
ade through leu
DNA between ade and attB
DNA between leu and attB
QUESTION 4
Using your deletion trick in Question 3, you indeed isolate a viable ?? ade+. Using a figure and words, show how you can now create a ?? that can transduce both ade+ and leu+. Hint: Draw a ?? ade+ chromosome next to a ?? leu+ chromosome. Please draw this out.
B C attP 1 2 3 cos coS ade attB leuExplanation / Answer
Based on the given data, question 1:
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