A transcription assay is performed with a plasmid that contains a known promoter

Question

A transcription assay is performed with a plasmid that contains a known promoter that produces a 400 base transcript. You have 8 microgram/ml of a 3.5 kb plasmid to serve as template in a 25 microliter reaction volume (DNA base pairs have a mass of 660 daltons). To a mixture of transcription factors and the other three NTPs you add alpha-32P-CTP which can be easily either as soluble radioctivity or TCA precipitable RNA transcripts. The transcripts accumulate linearly for about an hour and plateau at a total incorporation of 4.8 pMol of CTP.

a. How many templates were present in each reaction?

b. How many transcripts are produced in each reaction?

c. How many many transcripts per template are produced?

Explanation / Answer

Based on the given data,

a. Since, the total solution has two Plasmids, it has 2 templates

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