1. Many bacteria are naturally capable of taking up endogenousfragements of DNA
ID: 5417 • Letter: 1
Question
1. Many bacteria are naturally capable of taking up endogenousfragements of DNA in their environment, a phenomenon termed a. transformation b. conversion c. conjugation d. transfection e. hybridization 2. In addition to restriction enzymes, which enzyme isrequired to insert a fragment of DNA into a cloning vector? a. DNA polymerase b. RNA polymerase c. DNA ligase d. Topoisomerase e. Reverse transcriptase 3. When yeast cells are cultured in the presence of bothglucose and galactose, the GAL genes are a. transcribed but not translated b. neither transcribed nor translated c. transcribed and translated d. translated but not transcribed e. none of the above 4. The subfield of genomics that deals with gene expressionand interaction is a. comparative genomics b. expression genomics c. structural geneomics d. hypothetical genomics e. functional genomics 1. Many bacteria are naturally capable of taking up endogenousfragements of DNA in their environment, a phenomenon termed a. transformation b. conversion c. conjugation d. transfection e. hybridization 2. In addition to restriction enzymes, which enzyme isrequired to insert a fragment of DNA into a cloning vector? a. DNA polymerase b. RNA polymerase c. DNA ligase d. Topoisomerase e. Reverse transcriptase 3. When yeast cells are cultured in the presence of bothglucose and galactose, the GAL genes are a. transcribed but not translated b. neither transcribed nor translated c. transcribed and translated d. translated but not transcribed e. none of the above 4. The subfield of genomics that deals with gene expressionand interaction is a. comparative genomics b. expression genomics c. structural geneomics d. hypothetical genomics e. functional genomicsExplanation / Answer
2. the answer is DNA Ligase, because the restrictionendonuclease cuts the DNA, and the ligase re-attaches the new DNAfragment (or ligates it).
3. the answer is a, Transcription is just converting DNA toRNA by an RNA polymerase. The genes that are coded for in the RNAare often under the control of promoters and repressors. The Galgenes, code for Beta-galactosidase which cleaves lactose intoglucose and galactose. So if you have lots of glucose andgalactose, then it is because all of the lactose has been cleaved.When Lactose is absent in the cell the genes are repressed and youget no translation of the genes.
4.The answer is functional genomics
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