10. Which is NOT a direct step in determining the structure of a protein by x-ra

Question

10. Which is NOT a direct step in determining the structure of a protein by x-ray crystallography? a) Prepare a protein in a crystal form. b) Apply a constant magnetic field, and electromagnetic waves of varying frequency c) Purify a protein. d) Obtain a diffraction pattern. e) Determine the electron density map corresponding to the diffraction pattern observed. 11.Which level(s) of protein structure involve(s) hydrogen bonds between amino acid side chains? a) primary b) secondary and tertiary c) tertiary and quaternary d) secondary e) quaternary

Explanation / Answer

10) C

Protein purification. Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms.

11) Secondary structure

In long chain proteins there are regions in which the chains are organised into regular structures known as alpha-helices (alpha-helixes) and beta-pleated sheets. These are the secondary structures in proteins.

These secondary structures are held together by hydrogen bonds. These form as shown in the diagram between one of the lone pairs on an oxygen atom and the hydrogen attached to a nitrogen atom.

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