Hypothetical Chromatogram 8 Solvent Front line 7 6 5 4 -3 2 1 Spotting line 0 2)
ID: 540066 • Letter: H
Question
Hypothetical Chromatogram 8 Solvent Front line 7 6 5 4 -3 2 1 Spotting line 0 2) It takes about 4.78 L (microliters) of a copper solution containing 0.3193 g of copper ion per 100 mL to make a spot approximately 0.478 cm in diameter. Calculate the micrograms of copper ion in the spot. Using the "ruler" in the picture of the chromatogram and measuring with an accuracy of.1 unit, calculate the retardation factor (Ry) for compound.. MEASUREMENTS AND CALCULATIONS 1a) How far did compund C move? lb) At the same time, how far did the solvent move? units" "units".Explanation / Answer
1a.
initial position: 1.4 cm
final position: 2.4 cm
distance travelled = 2.4 - 1.4 = 1 cm
1b.
initial position: 1.4 cm
final position: 6.2 cm
distance travelled = 6.2 - 1.4 = 4.8 cm
1c.
Rf = distance by compound / distance by solvent
= 1/4.8 = 0.2083
Since it's a ratio, it has no units.
2.
100 mL contains 0.3193 g Cu
1 mL contains 0.3193/100 = 3.193*10-3 g Cu
4.78*10-3 mL contains 3.193*10-3 * 4.78*10-3 = 1.526254*10-5 g Cu
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