You wish to amplify the following double stranded DNA segment using PCR. The fol

Question

You wish to amplify the following double stranded DNA segment using PCR. The following sequence represents both strands. What are the sequences of the 2 oligonucleotide primers (5' to 3') that you would use to amplify this entire DNA segment? *Tm values of the primers should be similar.

5'-ACGTGCCTCTCCTCATACGAGGATATATATAGTGTTATTTTGTTTGGGGTGTTTGTTTTGG

ATTGTGTTTTTTTGTTTATTTCGTGTGTTATGTAACATATGACGTGCCTCTCCTCATATAATT

TCTATCTACTTGCACAATGAAATATATTCATAAAGCATAAGCTCATTG-3'

Antiparallel Strand: TGCACGGAGAGGAGTATGCTCCTATATATATCACAATAAAACAAACCCCACAAACAAAACCTAACA
CAAAAAAACAAATAAAGCACACAATACATTGTATACTGCACGGAGAGGAGTATATTAAAGATAGATGAA
CGTGTTACTTTATATAAGTATTTCGTATTCGAGTAAC

Explanation / Answer

The sequence of the primer for leading strand (anti parceled strand) will be 5' - ACGUGCCUGUCCUCAUAC-3'. For the lagging strand multiple primers will be used.

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