The lac operon consists of a promoter that initiates transcription of the gene i

Question

The lac operon consists of a promoter that initiates transcription of the gene i, which encodes a repressor protein. A separate promoter, p, within the operon initiates transcription of z, which encodes -galactosidase, and y, which encodes permease. The operator, o, where the repressor protein binds to inhibit transcription, is positioned between the promoter and the permease and -galactosidase genes. The superscript u denotes uninducible mutations, whereas superscript c denotes a constitutively active mutant. The table below describes the activity of -galactosidase and permease for haploid and diploid mutant strains.

Explanation / Answer

Strain 1 Cis regulation

Strain 2 Cis regulation

Strain 3 Trans regulation

Strain 4 Cis regulation

Strain 5 Trans regulation

Strain 6 Cannot determine

Permease and beta-galactodsidase are encoded by two genes which are described by the activity for haploid and diploid mutant strands. In haploid the mutation will cause high level of operon compared to the mutant in diploid.

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