Bile Esculin Remove the medium from the incubator and record the results in the
ID: 53576 • Letter: B
Question
Bile Esculin
Remove the medium from the incubator and record the results in the software for the Virtual Lab Report. Also record the results in the table below. If you need help interpreting the test, click on the “M” icon and to read up on how the medium is used. You can look up the tests for which it is used by clicking on the “T” icon and reading up on those tests.
Biochemical Tests
Staphylococcus aureus
Media Tests 5
Staphylococcus saprophyticus
Media Tests 6
Growth on Bile
Esculin Hydrolysis
Which of the following bacteria would be most likely to encounter bile salts in their normal habitat? (Circle or highlight all correct answers)
Staphylococcus aureus Enterobacter aerogenes Enterococcus faecalis
Streptococcus pyogenes Escherichia coli Staphylococcus epidermidis
Why would those you selected be expected to grow on bile esculin agar?
Where in the digestive system are lipids digested? Is there any evidence that these bacteria are more inclined to cause infections in those organs than are bacteria incapable of growing on bile?
It is possible to have an organism that is positive for both “growth on bile” and “esculin hydrolysis”, an organism that is negative for both, and an organism that is positive for one but not the other. Explain the only possible way an organism could be positive for one but not for the other when using this medium.
Biochemical Tests
Staphylococcus aureus
Media Tests 5
Staphylococcus saprophyticus
Media Tests 6
Growth on Bile
Esculin Hydrolysis
Explanation / Answer
Bile esculin agar test is performed to identify the organisms that can grow in the presence of bile salts based on heir ability to hydrolyse esculin. Gram negative bacilli, some gram positive cocci (such as enterococcal species), and staphylococcus species grow in the media containing bile salts. The following bacteria would be most likely to encounter bile salts in their normal habitat.
Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Staphylococcus epidermidis
The above selected species can hydrolyse esculin into esculetin and we can observe obvious growth of the organism, while the growing media is subjected to very dark discolouratoin.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.