A mixture of three proteins (A,B,C) can be theoretically separated by anion exch

Question

A mixture of three proteins (A,B,C) can be theoretically separated by anion exchange chromatography using cellulose coated with diethylamino-ethyl groups (pKa 10.5). In the first step the mixture was applied to resin at pH 7.5 and washed with the same buffer to elute protein(s) that did not bind. The resin was then washed with a buffer at pH 4.5

   Protein A is a homodimer with a molecular weight of 50 kDa and a pI of 3.0

   Protein B is a heterotetramer with a molecular weight of 100 kDa and a pI of 6.0

   Protein C s a monomer with a molecular weight of 75 kDa and a pI of 9.0

Which one of the following statements is incorrect?

a. Proteins A and B are initially bound to the resin at pH 7.5

b. The resin itself will remain positively charged over the pH range 3 to 7.5

c. Only protein A will elute when the resin is washed with a buffer at pH 4.5

d. Protein C does not bind to the resin at pH 7.5

Explanation / Answer

For the given 3 proteins

Separation by anion exchange resin,

Correct statement would be,

C. Only protein A will elute when the resin is washed with a buffer at pH 4.5

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