Which the following can be used to determine approximate molecular weight of a p

Question

Which the following can be used to determine approximate molecular weight of a protein? (Two correct answers - choose the best two) A) SDS-PAGE (polyacrylamide gel electrophoresis) B) PAGE. C) Size-Exclusion chromatography D) Ion-Exchange chromatography. E) ESI MS A student used a resin containing polystyrene (hydrophobic properties) and sulfonic groups (SO_3^-) for ion-exchange chromatography. Determine which will elute first using a pH 7.0 buffer for the following pairs. (___) Asp and Lys (___) Arg and Met (___) Gly and Leu (___) Ser and Ala Explain your answers above!

Explanation / Answer

a) For the determination of approximate molecular weight of protein is carried out by using the

1) SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis)

2) size exclusion chromatography

both techniues is used to determine the molecular weight of protein.

b) 1) asp will elute first because it is the least positive charge amino acid.

2) met will elute first because it is the least positive charge amino acid as compared to arg because of the the structure of them and arg have more charge than met so it will ellute first.

3) Leu will ellute first because Glycine is the proteinogenic amino acids.

4) ser will elute first because it is least positive charged than Ala.

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