As enzymes are purified, assays are performed to characterize the proteins: A) A
ID: 533352 • Letter: A
Question
As enzymes are purified, assays are performed to characterize the proteins: A) Activity B) Charges C) Size D) Affinity E) None of the above Which of the following is added to gel electrophoresis to denature proteins: a) Trypsin b) Sodium dodecyl sulfate (SDS) c) Salt and pepper d) None of the above Two-dimensional electrophoresis is a combination of which two techniques? a) isoelectric focusing and affinity chromatography b) ion-exchange chromatography and SDS-PAGE c) affinity chromatography and SDS-PAGE d) isoelectric focusing and SDS-PAGE e) isoelectric focusing and ion-exchange chromatography Affinity chromatography: a) allows high resolution and rapid separation b) separates proteins based on size. c) separates proteins based on charge. d) separates proteins based on attraction to another molecule. e) separates proteins based on charge and size.Explanation / Answer
Ans 22. A) Activity
Protein assays basically measures the concentration of protein in a solution.
Protein activity assays are performed for various disease's diagnosis , prognosis and treatment , such as cancer.
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