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This Table shows assay data from a purification procedure. Which statement is mo

ID: 533146 • Letter: T

Question

This Table shows assay data from a purification procedure. Which statement is most likely TRUE? The gel-filtration step increased the specific activity but not the yield. Of the three chromatography steps, the affinity column step had the biggest drop in yield. After the CM-cellulose step, the purification factor reached over 1000. indicating effective purification. Most of the protein removed in the gel- filtration step was likely enzyme that had unfolded during the CM-cellulose step. All of the above are false.

Explanation / Answer

Given the data for M4-HW-07,

the specific acivity and total protein content,

The true statement about purification procedure based on the given data would be,

A3. after the CM-cellulose step, the purification factor reaches over 1000, inidicating effecive purification.

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