molarity and volume of the M2V2, where Mi and Vi are the molarity concentrated s
ID: 532360 • Letter: M
Question
molarity and volume of the M2V2, where Mi and Vi are the molarity concentrated solution, respectively, and Mr and V are the and volume of the dilute solution. 3. a. The solution described in problem #2 is placed in the spectrophotometer. Based on the calibration curve shown on the next page, what would you predict the absorbance to be? Note: like the example given in the discussion, the absorbance should be calculated not just estimated from the graph. Notice that the slope and intercept of the calibration curve are provided in the figure on the next page. AbsorbanceExplanation / Answer
1. The relation between absorbance and concentration is given by
Absorbance= 2.1033*104* concentration +0.0039
given Absorbance= 0.311, hence 0.311= 2.1033*104* concentration +0.0039
concentration = (0.311-0.0039)/2.1033*104= 1.46*10-5M
there are 1.46*10-5 moles in 1 L or 1000ml
250 ml solution contains (250/1000)*1.46*10-5 moles of Red-40=3.65*10-6 moles
molar mass of Red-40= 496 g/mole
Mass of Red-40 = moles* molar mass= 3.65*10-6*496= 0.00181 gm
Mass % of red-40 in the sample= 100* mass of Red-40/total mass= 100*0.00181/ 0.2548=0.7105%
2) 250 ml of initial solution contains 11.22 gm
50 ml of this solution contains 11.22*50/250= 2.244 gm
2.244 gm of drinks mix will be there in the second 250ml solution.
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