15. PROPERTIES OF A CATION EXCHANGE RESIN-Fe UNKNOWN Introduction lon-exchange c
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15. PROPERTIES OF A CATION EXCHANGE RESIN-Fe UNKNOWN Introduction lon-exchange chromatography is used to exchange the ions in a solution (not just a catchy name!). The analyte ions in solution are attracted to ions covalently attached to stationary phase and replace whatever counter ion was previously attracted to the column material (stationary phase). The stationary phases used in ion-exchange chromatography may be divided into two types *cation-exchangers which contain negatively charged groups (e.g. -SO3) attached to the resin (usually polystyrene), attract positively charged ions which contain positively charged groups (e.g. -N(CH3) attached anion-exchangers to the resin, attract negatively charged ions N(CH3 Cl NO3 This experiment uses a simple gravity-flow column. The column will contain a cation exchange resin known as Dowex 50W-X2. Initially, the polymer is preconditioned to contain protons (H next to each negative site, producing many sulfonic acid groups solution of unknown Upon exposure of the resin to cations other than H" (such as a Fe protons are released into the mobile phase when the replace them, hence the term "cation exchange." The released protons are titrated with a base of known concentration and the concentration of the unknown cation can be determined Experimental Procedure A. Preparation of approximately 100 mL of 1 M HCl from the provided 6 M solution measure the appropriate volumes of HCl solution and Nanopure water using a graduated cylinder into a labeled beaker. Prepare-1Lof 0.02 MNaoH by dilution of 50 NaoH. Standardize your NaoH by titrating a solid primary standard at least four times (look back in your notebook, you have done something like this before) B. Preparation of stationary phase. Clamp a 0.7 cm x 15 cm glass column from the to a buret stand. This cylinder has a porous glass frit Ohaus balances drawers beneath t before the tip which keeps the resin from spilling out of the column. It also substantially reduces the flow rate of eluent through the column which is why we are using such a small amount of stationary phase. Check to make sure the column is not clogged by running DI water and a small amount of the hydrochloric acid through it weigh between 1.1 and 1.2 g of Dowex 50W-X2 resin into a small, clean beaker. Add 10 mL (graduated cylinder) 1 M HCI into the beaker with the resin and swirl well to e resin to the fully protonated condition saturated with all sulfonate groups on the resin). Carefull decant the excess acid from the beaker into approximately 10 mL of deionized water and swirl well. Decant the rinse water and repeat the water wash process 2x. Place the waste into the "DowexExplanation / Answer
First to thssi problem there´sno question defined.
What we can do is a write a basis of this type of analysis.
Ion exchange resisns are usually used to separate some dissoloutions that present some ions.
Following the text. We need to prepare some dissoloutions. First we need to dilute the HCl 6 M to have a 1 M.
This means if we need to prepare 100 mL, we have to dilute taking an aliquot to prepare the 1 M solution.
And we can do the same to prepare the NaOH solution. Having this we can proceed to do the next experiments.
Some calculations like retention percentage, resin retention, etc can calculate.
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