a. What is the function of the mobile phase for developing a chromatogram? b. Wh

Question

a. What is the function of the mobile phase for developing a chromatogram? b. What is the chemical composition of the mobile phase in this experiment? a. What is the function of the stationary phase for developing a chromatogram? b. What is the stationary phase in this experiment? a. What is the function of the eluent for developing a chromatogram? b. What is the eluent (eluting solution) in this experiment? Experimental Procedure, Part A. Distinguish between the functions of the developing chamber and the chamber. 5. Experimental Procedure, Parts A.4 and B.1. Paraphrase what must be done to prepare the stationary phase developing chamber in this experiment, Part B.3?

Explanation / Answer

1.

a. Chromatography is a method to separate the components between two phases.

The mobile phase carries the sample molecules along where as the stationary phase interacts with them. The stronger the interaction slower their movement. This differential interaction causes them separate out and elute at different times.

b. It depends on the type of chromatography.

In ion exchange chromatography aqueous salt solutions are generally used as mobile phases.

Normal phase chromatography the mobile phase typically nonpolar solvents such as hexane,heptane are used in combination with slightly more polar solvents is a propanol.

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