What is the principle of SDS-PAGE ? Why was the SDS-Sodium dodecyl sulfate used
ID: 521970 • Letter: W
Question
What is the principle of SDS-PAGE ? Why was the SDS-Sodium dodecyl sulfate used in the experiment? HOW would you estimate the mass of the protein using SDS PAGE? On the SDS-PAGE below, lane 1, label with the asterisk the band that would correspond to myoglobin, and explain why do you think this is this band. What else can you say about the protein sample in the lane 1? What is the color change of myoglobin when it Is oxidized and reduced? Explain with reasoning and confirm with experimental data. Why is olive oil liquid at room temperature? How do you make 2000 ml of a 5mM solution of Tyrosine? Molecular Weight: 181 2 g/mol. How do you make a dilution of the above solution to get a final concentration of 3 mM? I need a final volume of 1000 ml. Find at least 5 problems/mistakes on this figure Explain what they are and suggest how to fix them.Explanation / Answer
(2)
This is because the melting point of olive oil is 267 K, which means it will be liquid above this temperature, and also at room temperature.
(3)
Moles of tyrosine required = Molarity*Volume = 2*0.005 = 0.010 moles
Mass of tyrosine required = moles*MW = 0.010*181.2 = 1.812 g
So dissolve 1.812 g tyrosine in 2 L of water to make the desired solution.
(3)
Firstly, the regression equation ( linear fit ) is not a good fit, since it doesn't include most of the data points. So a better fit has to made for this case.
Second, in the plot it is shown that the slope is negative, while for a calibration plot the slope is always positive.
Third, the absorbance readings greater than 1 are considered absurd, so solutions should be diluted to such a conc that the absorbance should not exceed one.
Fourth, the conc readings along the x-axis are not evenly placed. The dilutions should be such that the data points are uniformly spread along the x-axis.
Fifth, negative absorbance readings also have no value, so extending the plot towards the -ve y-axis is absurd.
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