How would the proteins in the above table be separated from one another through

Question

How would the proteins in the above table be separated from one another through a cation exchanger column run at pH 7.0.
Clearly indicate the order in which the proteins would move through each separation technique from fastest to slowest (faster > slower) if any of the proteins will have a similar mobility through the separation technique then indicate this with a ~ symbol.
After working through this, i think the answer should be
Pie-2 > Pie-1 ~ Ytr-3 ~ Swc-1 > Kds-1
6. Use the table containing protein information in answering questions 6a-6c. Protein name Molecular weight Molecular weight Protein function pl (in kDa) as (in kDa) as determined by determined by SDS PAGE with Gel Filtration DTT treatment column cahan Chromatography Pie-1 tro) Enzyme and 6.9 114 220 binds to CAMP 114 5.4 Pie-2 220 Enzyme Kds 155 BYtr-3 30 90 Swc-1 8.00 neutral 73 Binds to pie-1 6.9 72 80 Binds to CAMP

Explanation / Answer

You can separate basing on the differences in molecular weight but Pie-1 and Pie-2 have
same molecular weights, lower the mol weight faster the protein will travel, even for cation
excahnger column
So the order will be,
Ytr-3 > Swc -1 > Pie-2 ~ Pie-1 > Kds -1

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