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The decomposition of hydrogen peroxide (substrate S) is catalyzed by the enzyme

ID: 520756 • Letter: T

Question

The decomposition of hydrogen peroxide (substrate S) is catalyzed by the enzyme catalase (E). At an initial catalase concentration [S_0] of 3.5 times 10^-9M the variation of 1/R_0 (the reciprocal of the initial rate) with 1/[s]_0. (the reciprocal of the initial concentration of the substrate) is given by (Lineweaver-Burk equation), (1/R_0) = 0.6977 s (1/[S]_0) + 26.557 s M based on this experimental result, which one of the following choices is correct? (left to right: Maximum rate R_max in M/s, Michaelis constant K_m in M and the turnover number k_2 in 1/s)

Explanation / Answer

compare 1 and 2 we get Km/Rmax= 0.6977s, 1/Rmax=26.55 s/M there fore Rmax=1/(26.55 s/M),

Rmax=0.0377 M/s substitue this value in Km/Rmax= 0.6977 gives Km=0.677*0.0377 M

Km=0.0263M then we know Rmax=K2*[So]

K2= Rmax/[So]

K2=0.0263/(3.5*10-9)

k2=1.08*1071/s

there fore option C is correct

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