Enolase has a strict requirement for two Mg2+ ions in its active site. Propose a

ID: 518153 • Letter: E

Question

Enolase has a strict requirement for two Mg2+ ions in its active site. Propose a role for these ions in the catalytic mechanism of the enzyme.

a)The two Mg2+ ions act as complexing agents and stabilize the tertiary structure of the enzyme.

b)The two Mg2+ ions neutralize the negative charges on the substrate and lower the pKa of the C2 proton, making it easier to abstract.

c)The two Mg2+ ions neutralize the negative charges on the substrate and reduce steric hindrance of the C2 proton, making it easier to abstract.

a)The two Mg2+ ions act as complexing agents and stabilize the tertiary structure of the enzyme.

b)The two Mg2+ ions neutralize the negative charges on the substrate and lower the pKa of the C2 proton, making it easier to abstract.

c)The two Mg2+ ions neutralize the negative charges on the substrate and reduce steric hindrance of the C2 proton, making it easier to abstract.

d)The two Mg2+ ions bind sulfide groups in active site which allow maintain optimal conformation of the enzyme.

Enolase requires two Mg2+ ions neutralize the negative charges on the substrate and lower the pKa of the C2 proton, making it easier to abstract.

Hence, the answer is B.

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