1. A student is making up a buffer solution to perform an experiment on an enzym
ID: 517766 • Letter: 1
Question
1. A student is making up a buffer solution to perform an experiment on an enzyme. She starts with an aqueous acetic acid solution in which the following equilibrium is established:
CH3COOH + H2O CH3COO- + H3O+
Next, she adds some sodium acetate, NaCH3COO to the solution.
Indicate whether you think each of the following statements is true or false.
A) There will be an increase in the concentration of acetic acid, CH3COOH
B) There will be no change in the pH
C) If a solution contains any amount of acetic acid and acetate ion, the solution is a buffer.
D) If a strong acid or base is added to a buffer solution, there is no change in pH.
E) The order of acid stregnths is ClCH2COOH < CH3COOH < HCOOH
F) It is more diffficult to remove the proton from acetic acid than from the carboxylic group of glycine, +NH3CH2COOH.
G) A protein with many acidic side chains can never have a net charge of zero.
Explanation / Answer
A) There will be an increase in the concentration of acetic acid, CH3COOH (TRUE)
B) There will be no change in the pH (FALSE)
C) If a solution contains any amount of acetic acid and acetate ion, the solution is a buffer. (TRUE)
D) If a strong acid or base is added to a buffer solution, there is no change in pH. (FALSE)
E) The order of acid stregnths is ClCH2COOH < CH3COOH < HCOOH (FALSE)
F) It is more diffficult to remove the proton from acetic acid than from the carboxylic group of glycine, +NH3CH2COOH. (FALSE)
G) A protein with many acidic side chains can never have a net charge of zero.(TRUE)
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