Hydrolysis of proteins in the body leads to the formation of individual ________

Question

Hydrolysis of proteins in the body leads to the formation of individual ___________s. Shown below are the structures of two co-enzymes, pyridoxine (vitamin B6) and pyridoxal phosphate (PLP). We can say that PLP is vitamin B6 which has been _______ ed and ________ed. In the first step of virtually all PLP-dependent reactions the aldehyde group of PLP forms an imine (Schiff Base) linkage with the NH_2 group of a lysine side chain of an enzyme (Step 1 below). Generally in the next step the N atom of an amino acid replaces the lysine N atom (Step 2 below). Overall, the PLP has undergone a trans-_________ ation reaction. The mechanism of the reaction referred to in question 3 above is shown below as described in our text. Match each of the numbered structures in the mechanism with their proper description from the list below. a. ________ PLP-amino acid imine b. ___________ PLP-enzyme imine c. _________ Geminal Diamine Intermediate which would eliminate amino acid d. ___________ Geminal Diamine Intermediate which would eliminate H_2 N - Enzyme e. ____ enzyme f. ________ amino acid

Explanation / Answer

Solution: Protein consists of precise sequence of amino acids that allows it to fold up into a particular three dimensional shape ,protein breakdown by hydrolysis involves breaking the peptide bonds through the addition of water to form a carboxlyic acid and an amine .It takes place at body temperature and is catalyzed by an enzyme .

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