1. During the extraction of spinach, you will shake the spinach with a mixture o
ID: 513569 • Letter: 1
Question
1. During the extraction of spinach, you will shake the spinach with a mixture of acetone, hexane and water. You will encounter the following two problems during the extraction. Explain what you would do to remedy that situation. (0.5 x 2 -1 point) tube when they mix (remember that it has immiscible liquids an organic liquid (acetone hexane) and water) b) Emulsion in the centrifuge tube during mixing 2. Draw a picture of a TLC plate of dimensions 10 cm (length) x 3.3 cm (width). Use a ruler to make your illustrations (0.5+0.5+2 3 points). Show the formula for R and your work to get full points. Mark the base-line at 1.6 cm from the bottom of the plate and mark the solvent front at 0.6 cm from the top of the plate. i. Make two circles corresponding to two spots on the plate. Make the first circle centered at 3 cm from the base-line corresponding to spot 1 and the second circle centered at 6 cm from the base- line corresponding to spot 2. i. Calculate the Rf of spot 1 and spot 2.Explanation / Answer
(a)
If you have more than 1.5 mL of acetone in the centrifuge tube at this point, it’s a good idea to reduce the volume via evaporation in a warm sand bath. Don’t forget the boiling chip or boiling stick or it will spatter. Allow the extract to cool to room temperature before the next step.
(b)
Water and hexane do not mix reality is that a little bit of the water will stay in the hexane. You can tell there is water in the hexane layer; it may be a little cloudy. You must dry the hexane layer by adding some anhydrous Na2SO4. A few microspatula scoops is usually sufficient. Na2SO4 soaks up the water as it becomes hydrated.
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