What is the major difference in scanning electron microscopy (SEM) when compared
ID: 50855 • Letter: W
Question
What is the major difference in scanning electron microscopy (SEM) when compared to light microscopy (LM) or transmission electron microscopy (TEM)?
What is the major difference in scanning electron microscopy (SEM) when compared to light microscopy (LM) or transmission electron microscopy (TEM)?
SEM is not good at viewing higher magnifications. The original image is in black and white. SEM uses whole, unsectioned surfaces that are covered in carbon and gold dust. SEM uses a beam of light to view specimens.Explanation / Answer
The right answer is choice (C) - SEM uses whole, unsectioned surfaces that are covered in carbon and gold dust.
SEM provides three dimensional pictures of whole, unsectioned surfaces with good striking clarity. The specimen that need to be viewed under SEM should be preserved and coated with fine layers of carbon and gold dust. An electron beam from SEM scans the specimen and causes other secondary electrons to be emitted. Then assembles all of them into a 3 dimensional image on cathode ray tube.
The original image is in black and white and it can be colored artificially to highligth structural details. This option is not choosen as right answer, as even TEM's original images are in black and white.
SEM do not use light beam, but uses electron beam. It is the light microscope that uses light beam to view specimens. Hence, the option (D) is incorrect.
SEM is good at viewing higher magnifications. It produces images with striking clarity. Therefore, the option (A) is incorrect.
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