The Michaelis-Menten equation is often used to describe the kinetic characterist

Question

The Michaelis-Menten equation is often used to describe the kinetic characteristics of an enzyme-cattily reaction. v = V_max [S]/K_m + [S] Where v is the velocity or rate, V_max is the maximum velocity, K_m is the Michaelis-Menten constant, and [S] is the substrate concentration. A graph of the Michaelis-Menten equation is a plot of a reaction's initial velocity (v_0) at different substrate concentrations ([S]). First, move the line labeled "V_max" to a position that represents the maximum velocity of the enzyme. Incorrect. Remember, V_max is the maximum velocity that an enzyme would achieve at high [S]. K_m is the concentration of substrate that gives 1/2 of this velocity. Estimate the values for these important constants: V_max = 175.0 mu M/min K_m = mu M

Explanation / Answer

Q1.

Note that, we must first state the VMAX, this is given at the Max point in the BLUE curve.

This is about 175 mM/min

Choose The RED LINE to be the Limit or top line of the BLUE LINE.

Q2.

Half VMax, is, by definition, the half of the Max velocity,

if we used 175 mM/min then --> Vmax/2 = 175 / 2 = 87.5 mM/min

The Orange horizontal line must go in the 87.5 mM/min level

Km will be the value in which this line crosses with the BLUIE LINE

which is about 12-14 mM of [S], best answer is in fact 13 mM as shown above

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