4. Given the below-delineated DNA fragment in which the sequence is provided for

Question

4. Given the below-delineated DNA fragment in which the sequence is provided for ONE of the two strands. Design forward and reverse 15mer primers that can be used to PCR amplify the underlined section.

      3’-ATGGAGATGGGCAGTAAAGGGGTGACG. . . 1500 nt. . . CCCGAGAACTTCACTGAGAGGGTCCC-5’

            Forward: 5’-   ___________________________________________-3’   

            Reverse: 5’-   ___________________________________________-3’   

Explanation / Answer

The forward and reverse polymer are 10 to 20 bp long so if we want to design a forward primer

Forward will be CTCTACCCGTCATTTCCCCACTGC

Reverse will be CCCGAGAACTTCACTGAGAGGG

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