1/ How did your actual gel results compare to your gel predictions? 2/Do you see
ID: 49665 • Letter: 1
Question
1/ How did your actual gel results compare to your gel predictions?
2/Do you see any bands that are not expected? What could explain the origin of these unexpected bands?
3/Does the gel show that your restriction digest and ligation procedures were successful? Describe the evidence you used to make this assessment.
4/In the geK– and geA– lanes, do you see evidence of multiple configurations of plasmids? Explain your answer.
5/In the geK+ and geA+ lanes, do you see evidence of complete digestion? Explain your answer.
6/In which lane would you expect to find the rfp gene and the ampR gene in the gel photograph? Are you able to locate these two genes? Explain your answer.
7/Compare the lanes that have linear fragments with the lanes that have plasmids. Is there a difference in the shape of the bands between these two DNA forms?
Explanation / Answer
ans 2. . yes there were unexpected bands , this may be because the primers annealed at multiple regions in the genome.
please label the lanes.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.