1.Why is it important to verify that you have the correct recombinant plasmid? 2
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Question
1.Why is it important to verify that you have the correct recombinant plasmid?
2.How did your actual gel results compare to your gel predictions?
3.Do you see any bands that are not expected? What could explain the origin of these unexpected bands?
4.Does the gel show that your restriction digest and ligation procedures were successful? Describe the evidence you used to make this assessment.
5.In the geK– and geA– lanes, do you see evidence of multiple configurations of plasmids? Explain your answer.
6.In the geK+ and geA+ lanes, do you see evidence of complete digestion? Explain your answer.
7.In which lane would you expect to find the rfp gene and the ampR gene in the gel photograph? Are you able to locate these two genes? Explain your answer.
8.Compare the lanes that have linear fragments with the lanes that have plasmids. Is there a difference in the shape of the bands between these two DNA forms?
9.In Laboratory 3, you described all the possible plasmids that you could make by ligating the digesting fragments of the pKAN-R and the pARA plasmids. Two of the rfp gene fragments (807 bp each) may form a circularized fragment because each end of the fragments terminates in BamHI and HindIII sticky ends. Is there evidence of a circularized 1,614 bp fragment in the geLIG tube lane? Explain your answer.
Explanation / Answer
answer 1 ---- It is important because , it is the only way to know for certain that correct genes were inserted into plasmid and that desired recombinant plasmid has been created.
for the rest of the answers please provide the image.
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