Enzyme inhibition. The Lineweaver-Burk plot (below) shows kinetic data for an un
ID: 49644 • Letter: E
Question
Enzyme inhibition. The Lineweaver-Burk plot (below) shows kinetic data for an unknown enzyme and inhibitor.
The reciprocal initial reaction velocity (1/v) is plotted as a function of the reciprocal substarte concentration
(1/[S]), in the absence or presence of inhibitor. The enzyme concentration was held constant at 1 M.
Question #3 (continued)
A) What is the type of inhibition?
B) Where does the inhibitor bind on the enzyme?
C) What effect does the inhibitor have on the substrate affinity for enzyme (KD)?
D) What effect does the inhibitor have on the rate of substrate conversion into product?
Explanation / Answer
enzyme inhibhitors will bind to the ezyme and prevent it wih binding to substrate . the inhibitors can bind to enzyme in reversible and non reversible manner , . based on their concentartion ,they can compete with the substrate and prevent it form binding to the enzyme . they can be competitive and non competitive . line weaver burk plots helps in distingush the types of inhibitors based on their competetion of binding to enzyme . they are double reciprocal plots .
a] the type of inhibhiton is non competetive inhibhiton
b] inhibhitor bind to the ezyme diffrent site other than active site and prevent substrate from binding
c] the binding of the inhibhitor changes the conformation of the enzyme , there by preventing it from binding to enzyme
d] the product formation decreases ,when inhibhitor concentartion increases .
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