HW1 Dr. Paul Cell Biology lecture 531 1. Which of the following steps in systema
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HW1 Dr. Paul Cell Biology lecture 531 1. Which of the following steps in systematic evolution of ligands by exponential enrichment (SELEX) enhances the diversity of product RNA aptamers? B. recovery of RNAaptamers with the desired ligand-binding property c. PCR amplification of RNA aptamers with an error-prone polymerase a. selection of RNA aptamers with the desired ligand-bmdmg property d. cloning of the selected RNA aptamers after completing successive rounds of SELEX 2. The protein moiety of the ribozyme Rnase P A. contains the active site for tRNA precursor cleavage B. serves as a shield to preventrepulsion between negative charges on the RNA moiety of RNase P and negative charges on tRNA precursors serves as the catalyst for cleavage oftRNA precursors in the presence ofthe RNAmoiety serves as the catalyst for cleavage oftRNA precursors in the absence ofthe RNA moiety 3.Which one of the following reactions is NOT catalyzed by a ribozyme? during transcription ie, synthesis of complementary RNA from DNA) C. D. A. formation of phosphodiester bonds B. cleavage ofprecursor RNAs into mature tRNAs C. removal of imtrons durimg trans-esterification reactions involved in certain mRNA splicng D. fomation of peptide bonds during protein synthesis 4. Which one of the following statements about the structure of RNA is NOT true? A. RNA is capable offorming secondary structure RNA is capable offormingtertiary structure RNA is capable offorming pseudoknots. RNA is capable offorming -sheets Murine, leukemia virus(MLV) uses a pseudoknot as a regulatory mechanism for transcription ofthe Gag-Pol mRNA processing ofthe Gag-Pol mRNA transport ofthe Gag-Pol mRNA translation of Gag protein versus the B. C. D. A. B. C. D. Gag-Pol fusion proteinExplanation / Answer
1. d is the right option, as after completion of selex process there is cloning of RNA molecules which starts so it is the right answer
2 b is the right answer. this i basically explained in terms of forces between charges whcih option b completely demonstrate
3 rna splicing is not done by ribozymes
4 rna cant for b sheets because of steric limitations
5. option d is the right answer. it affect at the initiation of translation
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