In an effort to study protein interactions, samples of protein X and a mutant fo

Question

In an effort to study protein interactions, samples of protein X and a mutant form of protein X are both washed through the same gel-filtration column. The mutant protein X runs through the column at a much slower rate than the normal version of protein X. Which of the following changes in the mutant protein could explain this result?

The answer is not A or C. So its either B or D.

A. The mutant protein X is both smaller and positively charged versus the normal protein X which is larger and negatively charged

B. The mutation causes the mutant protein X to be larger than the normal protein X

C. The mutation has changed the overall charge from negative to positive

D. The mutant protein X has lost the ability to form the dimers that the normal protein X forms

Explanation / Answer

B) The mutation causes the protein X to be larger than the normal protein X

Gel filtration column separates the proteins or a sample based on size,

as the mutant protein X runs through the column at a slower rate, its size should have definitely increased compared to the normal protein X

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