You have checked the size of monomeric Enc on SDS-PAGE; however, you are still u

Question

You have checked the size of monomeric Enc on SDS-PAGE; however, you are still unsure about whether or not the protein you see on the gel is your actual protein. From the following options, what is the best way of being able to conclusively determine the identity of your protein?

a Affinity chromatography and size-exclusion

b Size-exclusion and ion exchange

c Trypsin digestion and tandem MS/MS

d 2D-Gel electrophoresis and MALDI-TOF MS

I think the right answer is between c and d because size-exclusion is not necessary.

Explanation / Answer

its correct ans is d. because 2D Gel electrophoresis separate on the basis of Isoelectric point of protein if protein present there it will easily recognise by this technique and by the  MALDI-TOF MS techniques we can easily detect the molecular mass of protein so its correct answer is D.2D-Gel electrophoresis and MALDI-TOF MS

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