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Many cloning vectors carry a gene conferring resistance to the antibiotic ampici

ID: 48849 • Letter: M

Question

Many cloning vectors carry a gene conferring resistance to the antibiotic ampicillin as a selectable marker. Why is the ampicillin resistance gene called a selectable marker?

Bacterial cells must retain the vector in order to survive in the presence of ampicillin

You can be certain your vector is carrying a DNA insert when the cells are grown in the presence of ampicillin

Bacterial cells carrying the vector will only grow in the presence of ampicillin

Bacterial cells carrying the vector die in the presence of ampicillin

Explanation / Answer

Bacterial cells must retain the vector in order to survive in the presence of ampicillin.

Ampicillin resistance plasmid vectors allows the bacteria or any other cells to grow the presence of ampicillin. Bacteria those that are lack of this plasmid will die in th epresence of ampicillin due to lack of resistance.

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