You would like to know more about how the rR promoter works. Given the backgroun

Question

You would like to know more about how the rR promoter works. Given the background information about the DNA binding protein FIS (see Figure 3 and the accompanying text), write a hypothesis that addresses the effect of FIS binding sites (and by extension, FIS binding) on transcription activity of the rR promoter. For reminder, a DNA binding protein may serve as an activator or repressor of transcription, or it may have no effect at all.

A promoter that makes ribosomal RNA RNA polymerase binding site or FIS FIS FIS +1 .88 -50 41 +50 Figure 3. The promoter. The start site of transcription (indicating the first nucleotide acting as template for RNA) is labeled +1, and the direction of transcription is indicated by the bent arrow. A DNA binding protein called FIS (Factor for Inversion Stimulation, blue circles) interacts with DNA at site A (site A is part of the promoter DNA). The numbers below the promoter represent distance in base pairs from the transcription start site

Explanation / Answer

FIS is an important protein which regulates expression of other genes . it regulates its own expression , most abundant DNA binding protein , it is a global regulator protein in E.coli that activates the rRNA transcription by binding to three upstream activation sites of the rRNA promoter and enhances the transcription to 5 to 10 fold in vivo

it has a direct role in upstream activation of rRNA promoters .

Related Questions

Navigate

• Browse (All)
• Browse Y
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.