how do i calculate [HIn]equil ? i have [H3O+] intit , [In-]equil, measured pH va

Question

how do i calculate [HIn]equil ? i have [H3O+] intit , [In-]equil, measured pH values, and A550 values, with the information that i have how can i find the [HIn]equil of each of my samples? how do i calculate [HIn]equil ? i have [H3O+] intit , [In-]equil, measured pH values, and A550 values, with the information that i have how can i find the [HIn]equil of each of my samples? how do i calculate [HIn]equil ? i have [H3O+] intit , [In-]equil, measured pH values, and A550 values, with the information that i have how can i find the [HIn]equil of each of my samples?

Explanation / Answer

The equation for dissociation would be,

HIn <==> H+ + In-

So,

Ka = [H+][In-]/[HIn]

with equilibrium values for,

[In-] and [H+] and Ka value,

we can easily fin the left [HIn] in solution

then, add [HIn] found + [H+] to get original concentration of [HIn] in solution

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