1. Write down the equation from the trendline fit of the standard curve data tha

Question

1. Write down the equation from the trendline fit of the standard curve data that will be used to calculate the concentrations of the unknwon.

2. Based on the trendline fit, what is the extinction coefficient (e) of the egg albumin?

3. Determination of the concentration of unknown dilute samples using the trendline fit.
Show an example calculation:

4. Determination of the concentration of the unknown stock sample:
Show an example calculation:

Phvor Table Recommended lable Pictures online shapes Graph Recommended People Graph Recommended Pivot Tables Pictures screenshot. a My Add-ins Charts J sr. Maps PivotChart 3D Line Colum Tables Illustrations Add-ins Charts F18 Group D Group C 2 0 -0.0006 0 -0.00023 0.2 0.4128 0.2 0.136445 A280 Standard Data y 1.0254x+0.1048, 0.4 0.5615 0.4 0.352274 1.0000 0.6 0.7225 0.6 0.470312 0.8 0.8957 0.8 0.618789 1 0.8000 1.1130 1 1.080022 y 0.9952x -0.0547 0.8 [D] 10 [A] 0.6000 Group D 0.569 0.675 [E1 11 [B] Group C 0.45 0.4000 12 [C] 0.76 [F] 0.097 Linear (Group D) 13 Linear (Group 0.2000 0.0000 16 0.2 0.4 0.6 0.8 -0.2000 Concentration (mg/mL) 19 23 25 29 A820 Biuret Ready O Ask me anything

Explanation / Answer

1. As the graph for Group C fits nicely for dilute samples, ( less deviation for dilute samples from trendline), the equation for line used for dilute samples will be y = 0.9952x -0.0547

Similarly, Group D fits nicely for higher concentrated samples, as less deviation from trendline at higher mg/mL, so equation of trendline to be used at higher concentration is y = 1.0254x + 0.1048

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