6) Which of the following statementes regarding Sanger sequencing method is accu
ID: 48239 • Letter: 6
Question
6) Which of the following statementes regarding Sanger sequencing method is accurate? READ CAREFULLY!
a) This method is used to determine the amino acid sequences of a template DNA
b) A typical automated sequencing reaction using fluorescence consists of a DNA template, four dNTPs, a primer, buffer components, DNA polymerase, and four different fluorescently labeled ddNTPs.
c) DNA sequencing is essentially an in vitro translation reaction
d) The sanger sequencing method works because DNA polymerase can add a nucleotide onto the 3' end of a ddNTP.
7) The first step in positional cloning which involves analysis of a pedigree and the identification of polymorphic DNA markers that are inherited with a disease is referred to as:
a) reverse genetics
b) linkage analysis
c) Southern blotting
d) molecular cloning
8) Which of the following is an accurate description of a plasmid vector?
a) Prior to cloning an insert into a plasmid vector, the plasmid should be cut with scissors
b) A plasmid vector is a single stranded linear RNA molecule that contains a cloning site, a selectable marker, and origin of replication
c) A plasmid vector isn't useful for molecular cloning
d) A plasmid vector is a double stranded circular DNA that contains a cloning site, a selectable marker, and origin of replication.
Explanation / Answer
6. The statemente regarding Sanger sequencing method that is accurate is: b) A typical automated sequencing reaction using fluorescence consists of a DNA template, four dNTPs, a primer, buffer components, DNA polymerase, and four different fluorescently labeled ddNTPs.
7. he first step in positional cloning which involves analysis of a pedigree and the identification of polymorphic DNA markers that are inherited with a disease is referred to as: b) linkage analysis
8. an accurate description of a plasmid vector is: d) A plasmid vector is a double stranded circular DNA that contains a cloning site, a selectable marker, and origin of replication.
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