Starting with a 500 mg/mL saline stock solution, map out how you would create th

Question

Starting with a 500 mg/mL saline stock solution, map out how you would create the following dilutions, using water as your diluent. Start with the stock solution for each dilution what is the dilution factor or dilution ratio relative to the original solution for each at (he solutions you made? EACH OF THE DILUTIONS SHOULD HAVE A TOTAL FINAL YOU ME OF 10 mL AND THE DILUENT IS WATER. Example: 1.0 mg/mL 1^st Use C_1V_1 = C_2V_2 to solve for V_1 V_1 = (C_2/C_1) V_2= (1mg/ml/500mg/ml) * 10ml = 0.02 ml V_1 = (Dilution Ratio) V_2^nd Solve for V_DILUENT = V_2 - V_1 = 10ml middot 0.02 ml = 9.98 ml Write Recipe: 0.02 ml stock + 9.98 ml water Reduce the Dilution Ratio: 1/500 or 1:500 (Dilution Ratio is determined by taking C_2/C_1. but by convention is expressed with I in the numerator, so this sometimes has to be adjusted or reduced to express it this way) 250.0 mg/mL 75.0 mg/mL 20.0 mg/mL 10.0 mg/mL

Explanation / Answer

a)solution is 10 mg/ml

250mg/ml, V1= (250/500)*10 = 5 ml, stock solution = 5ml and water =10-5= 5ml

dilution ratio = 10/5 =2

b) solution is 10 mg/ml, V1= (75/500)*10 = 1.5ml, water =10-1.5= 8.5 ml

dilution ratio = 10/1.5 =6.6

c) solution is 10 mg/ml, V1 = 10*20/500 = 0.4ml, water =10-0.4 =9.6ml

dilution ratio =10/0.4= 25

d) solution is 10 mg/ml, V1= 10*10/500 = 0.2 ml, water = 10-0.2=9.8 ml

dilution ratio =10/0.2=50

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