Iso penicillin N (C_14H_21N_3O_6S) is produced by the fungi Penicillium Chrysoge

Question

Iso penicillin N (C_14H_21N_3O_6S) is produced by the fungi Penicillium Chrysogenum, This compound may be converted by the enzyme pen transacetylase to Pencillian G (Benzyl penicillin- C_14H_21N_3O_6S)by the same microorganism if the growth medium is supplied with phenyl acetic acid (C_8H_8O_2). The net reaction is lso penicillin N phenyl acetic acid rightarrow Pencillian G + Amino adipic acid 12.0 g of phenyl acetic acid is added to a broth containing 15.0 g of lso penicillin N What is the limiting reagent? If the yield based on the moles of limiting reagent fed is 7s%, what mass of benzyl penicillin is produced in the conversion?

Explanation / Answer

Mass of iso pencillin N = 15.0 g

Molar mass of iso pencillin N = 359 g/mol

moles of iso pencillin N = 15.0 g / 359 g/mol == 0.041 mol

Mass of phenyl acetic acid = 12.0 g

Molar mass of phenyl acetic acid = 136 g/mol

Moles of phenyl acetic acid = 12.0 /136 = 0.088 mol

According to reaction 1 mole phenyl acetic acid reacts with 1 mole of iso pencillin N .

Here we have less amont of iso pencillin N ..So, iso pencillin N . is limiting reagent

2)

So moles of benzyl pencillin == 0.75 x 0.041 mol == 0.03075 moles

Molar mass of benzyl pencillin = 334 g/mol

Mass of benzyl pencillin = 0.03075 moles x 334 g/mol == 10.27 g

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