Which of the following globular (or ellipsoid) proteins would elute first (in th

Question

Which of the following globular (or ellipsoid) proteins would elute first (in the void volume) from the following gel-filtration columns? Select all that apply.

Bio-Gel P-30 Bio-Gel® P-60 Bio-Gel P-100 exclusion limit of 40 kDa exclusion limit of 60 kDa exclusion limit of 100 kDa amylase (54,000 Da) a-amylase (54,000 Da) a-amylase (54,000 Da) B-amylase (210,000 Da) O Bamylase (210,000 Da) B-amylase (210,000 Da) lysozyme (15,000 Da) lysozyme (15,000 D O lysozyme (15,000 Da) lingual lipase (52,000 Da) O ngual lipase (52,000 Da) O ngual lipase (52,000 Da) standard (29,000 Da) standard (29,000 Da) standard (29,000 Da) lactoferrin (75,000 Da) O lactoferrin (75,000 Da) actoferrin (75,000 Da)

Explanation / Answer

Elution order in gel-filtration

Bio-gel P-30 (exclusion limit of 40 kDa)

lysozyme (15,000 kDa)

standard (29,000 kDa)

Bio-gel P-60 (exclusion limit of 60 kDa)

lysozyme (15,000 kDa)

standard (29,000 kDa)

lingual lipase (52,000 kDa)

alpha-amylase (54,000 kDa)

would elute first

Bio-gel P-100 (Exclusion limit of 100 kDa)

lysozyme (15,000 kDa)

standard (29,000 kDa)

lingual lipase (52,000 kDa)

alpha-amylase (54,000 kDa)

lactoferrin (75,000 kDa)

would elute first

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